Individual variation in proliferative potential of human diploid fibroblastsIn vitro

Abstract

The study of human diploid cells in vitro has established the identical values of parameters of proliferative potential in monozygotic twins and their significant difference in dizygotic twins [4]~ a decrease in the proliferative potential of cells obtained from patients with hereditary diseases, the principal phenotypic manifestations of which are signs of premature aging [2], and negative correlation between the proliferative potential of cells and the donor's age [6]. These findings are evidence in support of genetically determined control over this parameter and ~he presence of correlation between the proliferative potential of cells in vitro and in vivo. The percentage of cells capable of forming colonies consisting of 16 cells or more is a reliable indicator of the proliferative potential of a strain [7]. Of all strains studied in this respect only two are embryonic, and they have been obtained from lung tissue (strains Wi-38 and IMR-90). There are some doubts about the validity of comparing these strains with postnatal strains obtained from skin biopsy material [5]. There is sufficient information in the literature on the character of proliferation of human fibroblasts in vitro in connection with a study of aging problems. Results obtained by the study of each group are often a~eraged, and for that reason there are no data on individual variation within each group of donors.

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